Abstract: Qinghai gold-antimony ore is characterized by main recyclable elements Au and Sb, main recyclable mineral Stibnite and pyrite, fine disseminated grain-size of gold, and gold mainly wrapped in pyrite, and Au grade 3.50 g/t, Sb grade 1.95%.The experimental study on flotation of gold from the run-of-mine was carried out.The results showed that, at the grinding fineness of -0.074 mm occupying 80.32%, Au coarse concentrate re-grinding fineness of -0.037 mm occupying 95.45%, through one roughing-two scavenging-three cleaning closed-circuit flotation process, gold concentrate with yield of 4.01%, Au grade of 74.46 g/t, Sb grade of 4.19%, Au recovery of 85.31%，Sb recovery of 8.62%; tailings with yield of 95.99%, Sb grade of 1.86%, Au grade of 0.54 g/t, Sb recovery of 91.38%, Au recovery of 14.69% was obtained, Which realized pre-flotation of gold from the gold-antimony ore and material preparation for flotation ofantimonyfrom the remainder tailings.