Abstract: The Implementation Rules for Safety Assessment in Operation of Coal Mine is of great significance to perfect the coal mine safety evaluation system, prevent and contain serious accidents and improve the level of coal mine safety management. In order to promote the understanding and mastery of the Implementation Rules for Safety Assessment in Operation of Coal Mine by coal mine enterprises, safety assessment institutions and relevant units, to better play the role of the Implementation Rules for Safety Assessment in Operation of Coal Mine, firstly introduces the formulation background of the Implementation Rules for Safety Assessment in Operation of Coal Mine , emphatically expounds the main contents, it includes the scope of application, preliminary preparation, collection of data and site investigation, identification and analysis of hazardous factors, division of evaluation units, selection of evaluation methods, qualitative and quantitative evaluation, suggestions on safety measures, conclusions of safety evaluation and compilation of safety status evaluation report. Secondly, in view of the doubts existing in the implementation process of the Implementation Rules for Safety Assessment in Operation of Coal Mine, the evaluation report compilation unit, evaluation cycle, evaluation personnel, the specific evaluation content of each unit, accident statistical analysis and so on are analyzed in detail. Finally, from the three aspects of coal mine safety supervision department, coal mine enterprises and safety assessment institutions, puts forward some suggestions on the implementation, training and application of the Implementation Rules for Safety Assessment in Operation of Coal Mine.