Abstract: A low grade copper-lead-zinc polymetallic sulfide ore is taken as the research object. The process mineralogy and beneficiation experiments are carried out. The main recyclable elements in raw ore are copper, lead, zinc and sulfur. Among them, copper, lead and zinc exist in the form of sulfide, and sulfur mainly exists in the form of pyrrhotite. Firstly, comparative tests of partial mixed preferential flotation and preferential flotation processes are carried out. Through data comparison, the roughing condition test and open circuit test are carried out to optimize the flotation conditions and process structure using the preferential flotation process. On this basis, closed-circuit tests are carried out, and the results show that: under the condition of grinding fineness of −0.074 mm accounting for 80%, copper concentrate with copper grade of 24.90% and copper recovery of 85.62% can be obtained by “2 times roughing and 4 times cleaning”, lead flotation with “1 times roughing and 4 times cleaning and 1 sweeping”, zinc flotation with “1 times roughing and 3 times cleaning” and sulfur flotation with “3 times cleaning”. Lead concentrate with lead grade of 61.84% and recovery rate of 87.93%; zinc concentrate zinc grade of 55.11%, zinc recovery of 87.52%; The sulfur concentrate has a sulfur grade of 37.65% and a sulfur recovery rate of 74.06%. The research can provide reasonable process flow and parameters for the development and utilization of the ore, realize the comprehensive recovery of various valuable metal elements, and also provide reference and basis for the development and utilization of the same type of low-grade copper-lead-zinc polymetallic sulfide ore.