Abstract: The graphite ore from Gansu belongs to flake graphite ore,with low fixed carbon content of 4.48% and the flake of graphite is small.A beneficiation test of graphite ore from local area was studied and concluded the best rougher flotation conditions:fineness of rougher grinding accounted for 63.52% of -0.074mm,flotation concentration is 27%,the dosage of kerosene is 640g/t,the dosage of alpha-terpineol is 110g/t and the dosage of quicklime is 2500g/t(pH=9).An open and closed trial was conducted by five-times-regrinding and six-times-cleaning on the basic of the best rougher flotation conditions.Finally,the flow can obtain the graphite concentrate with the fixed carbon content of 95.70% and the recovery rate of 71.78%,providing a technical basis for explosion of graphite resources in the local.