Abstract: The assessment of adsorbed gas content is the key for the shale gas resource prediction and target area selection. The total content of organic carbon (TOC), kerogen maturity (R_o), temperature (T) and pressure (P) are the main factors which affect the adsorbed gas content(V), with the increase of strata depth (H), T and P increase at the same time, therefore, H reacts the mutual effect of T and P. Based on the isothermal adsorption experiment results, calculation model considering TOC, R_o, H are established, and influence effect and sensitivity are analyzed using orthogonal analysis method, the results show that: V is proportional to TOC, R_o, and there is a depth turning point between V and H. Sensitivity ranks of factors are H> TOC> R_o, and above the depth turning point, pressure is more sensitive than temperature, while below this point, temperature is more sensitive than pressure. The calculation model can assess any shale gas resource under different H, TOC, R_o; and the analysis method can also provide the basis for parameter optimization in shale gas reservoir.