Abstract: In this paper the wast beer yeast was used as biosorbent for the biosorption of Cu²⁺ for exploiting a new type of biosorbent.The biosorption action of Cu²⁺ was studied with waste beer yeast by the orthogonal method in diferent temperature、time、pH,initial copperion density and yeast density condition.The results show that the removal rate of Cu²⁺ can be over 92% under the condition of the adsorption temperature is 25℃,adsorption time is 90 min,value of pH is 5,the mass concentration of yeast is 2 g·L^-1 and the concentration of Cu²⁺ is 40mg·L^-1.The technique is simple,the wastewater is harnessed by other waste.Therefore,it will have better applied prospect.