Abstract: This paper discusses the separation mechanism and the advantages of using cyclonic static microbubble flotation column to gold ore separation,which included middling cycle,microbubble flotation and static flotation . When used in 8000t/d capacity concentrator of Sanshandao gold mine grade of gold concentrate over 50g/t was achieveed . The Industrial application results showed that the cyclonic static microbubble flotation column had good effect on gold separation.Meanwhile,several measures included permeating timely bubble generator when the contents of blockage reached a certain quantities ,reaching the design requirement fineness(-200 mesh was over 50%) ,optimizing the operating parameters and adding spray water which could improve flotation performance were also suggested