Abstract: Acid leaching, bioleaching were used to remove phosphorus from a high-phosphorus iron ore in western Hubei (iron grade 43.50%, phosphorus content 0.85%). The results showed that: under the condition of 2% pulp density, with 100ml single 0.1mol/L of oxalic acid (C₂H₂O₄), citric acid (C₆H₈O₇), H₂SO₄, HNO₃, HCl, the inorganic acid was better than organic acid, in which H₂SO₄ was the most effective, and the iron grade, recovery, and phosphorus removal rate were 49.08%, 99.57%, and 93.91%, respectively; in the mixed acid leaching of oxalic acid and citric acid, with the ratio between the range of 100∶0 ~ 20∶80, the phosphorus removal rate was accepted; when the pulp density was less than 5%, with the sulfuric acid leaching, the phosphorus content in the treated ore was 0.18%;In the bioleaching tests, the pulp density was 2%, with the Acidophilic Thiobacillus ferrooxidans (At.f) and the Aspergillus niger to treat the iron ore, the phosphorus content was reduced to 0.25% and 0.22%, respectively.