Abstract: In the paper, the authors give a comprehensive summary of the basic situation of the bioreagents, such as biocollector, biodepressant and bioflocculant, with the development of microbial flotation in the recent years. The paper focuses on the core issue of "Bioflotation", completely summarizing the usage condition of several special strains on the related minerals. Finally, the disadvantages of the present research and forcasts about the research direction, as well as the development tendencies about the microbial flotation are pointed out in the paper.