Abstract: To serpentine pure mineral as research object,pure mineral floatation tests,collecting agent(potassium butyl xanthate) adsorption measurement and solution chemistry analysis of related systems have been made.The results show that:Cu(II) and Ni(II) could effectively activate potassium butyl xanthate floatation serpentine as solution pH>6,disodium EDTA could effectively eliminate the activation;potassium butyl xanthate hardly couldn’t adsorption at the surface of clean serpentine,the adsorption of potassium butyl xanthate on the surface of serpentine could be improved greatly by Cu(II) and Ni(II),disodium EDTA could cut it down;in the range of pH 9~10,Cu(OH)₂(s) and Ni(OH)₂(s) are the main components in Cu(II) and Ni(II) solution,which will cover up serpentine and activate the floatation,the copper and nickel hydroxy complex could be complexed and stabilized by disodium EDTA,their content in solution could be cut down,thereby,the potassium butyl xanthate floatation serpentine could be inhibited.