Abstract: This paper takes typical Jianhe Hot Spring in Guizhou metamorphic rock area as the research object,starts from the geological and geothermal background of the hot spring area and analyzes the hydrogeochemical,hydrogen and oxygen isotopes characteristics of Jianhe Hot Spring.The results show that Jianhe Hot Spring has the characteristics of fracture (fissure) Hot Spring.The hot spring has no magma heat source,so it mainly relies on the earth heat flow in normal areas to provide heat and survive.The source of replenishment is meteoric waters.The elevation of Jianhe replenishment area is between 1050 and 1150meters.The geothermal reservoir temperature is 68.3℃.The circulation depth of geothermal water is about 2890 m.The formation of hot spring is mainly controlled by lithology and regional faults.Meteoric waters permeate and migrate downward through the fractured zone,gets heated continuously and rises till the Gedong Fault.They emerge in low-lying places and forms hot spring.