Abstract: Exploring the external environment and favorable conditions of microbial degradation of methane efficiently,was the foundation works for further optimization and formation the biological methods to controlling coal seam gas disaster.The pressure of the gas mixture and the oxygen concentration was adjusted by self-made device that could mix methane and oxygen in high-pressure.Then the device was connected with the adsorption tank after mixing completely.The variation characteristics of methane adsorption in coal samples in adsorption tank were observed.By spraying different concentrations of methanotrophic solution into multi-group dry coal samples placed in adsorption tanks in advance,changes of methane adsorption(desorption)in coal samples after adding methanotrophs and the content of carbon dioxide were analyzed deeply and meticulously under the conditions of high pressure and low oxygen supplied.The experimental results showed that biological respiration of methanotrophs was still existed under the conditions of high-pressure and low-oxygen.Increasing the pressure of gas mixtures,or enhancing the concentration of oxygen in gas mixtures,or raising the density of methanotrophs solution were in favor of degradation reaction.