Abstract: The disseminated grain size was generally fine of Zhejiang refractory fluorite ore, fluorite mixed with gangue minerals and inclusion with them each other. By the research on fluorite ore properties, a beneficiation test was carried out. The grinding fineness and reagent system was determined through rough separation and selection condition test, then, open and closed flotation circuit tests were carried out. The results showed that, acid grade fluorspar concentrate which CaF₂ content was 97.15% and recovery was 65.46% can be obtained through one roughing-eight cleaning, abandon first roughing, middlings Ⅱ~middlings Ⅷ sent back to grinding process. The middlings Ⅰ through regrinding and recleaning can obtain metallurgical quality grade fluorite which CaF₂ content was 81.32% and recovery was 82.48%. The research provided technical basis for utilization of fluorite ore in Zhejiang.